Abstract
p53 has been reported to repress a number of TATA-containing promoters in transient transfection assays. TATA-less promoters are generally believed to be refractive to p53 repression. We report here that the TATA-less promoters of Ets-family genes (Ets-1 and Ets-2) are efficiently repressed by wild-type but not mutant p53 in transient co-transfection assays. Moreover, p53 was immunologically detected in protein complexes formed on oligonucleotides from both the TATA-containing and TATA-less promoters. Our data suggest that p53 is involved in the regulation of the expression of both promoter types, most probably by protein-protein interaction. A model for p53 function in promoter repression is proposed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA-Binding Proteins*
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Down-Regulation
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Gene Expression Regulation
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HeLa Cells
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Humans
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Promoter Regions, Genetic*
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-ets
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Repressor Proteins / analysis
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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TATA Box*
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Trans-Activators / genetics
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Trans-Activators / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transfection
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Tumor Suppressor Protein p53 / analysis
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Tumor Suppressor Protein p53 / genetics
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Tumor Suppressor Protein p53 / physiology*
Substances
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DNA-Binding Proteins
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ERF protein, human
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ETS1 protein, human
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ETS2 protein, human
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Proto-Oncogene Protein c-ets-1
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Proto-Oncogene Protein c-ets-2
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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Tumor Suppressor Protein p53