Abstract
ZAP-70 is a protein tyrosine kinase (PTK) that plays a critical role in T cell activation. To study the role of ZAP-70 catalytic activity in this process, a substrate capable of distinguishing between the activities of ZAP-70 and other PTKs would be useful, especially since it has recently been shown that ZAP-70 interacts with another T cell PTK, Lck. We have thus identified a site of phosphorylation on the cytoplasmic fragment of the erythrocyte band 3 protein that is recognized by ZAP-70, but not Lck. A synthetic peptide based on this site has been demonstrated to be a good in vitro substrate for ZAP-70 and a poor substrate for the T cell PTKs Lck and Itk. This peptide molecule should thus prove useful to many investigators working in the field of T cell activation.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Anion Exchange Protein 1, Erythrocyte / chemistry
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Anion Exchange Protein 1, Erythrocyte / metabolism*
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Chromatography, High Pressure Liquid
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Humans
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
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Mass Spectrometry
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Myelin Basic Protein / metabolism
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism*
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Phosphorylation
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Protein-Tyrosine Kinases / metabolism*
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Receptors, Antigen, T-Cell / metabolism
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Recombinant Proteins / metabolism
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Substrate Specificity
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T-Lymphocytes / enzymology*
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ZAP-70 Protein-Tyrosine Kinase
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src-Family Kinases / metabolism
Substances
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Anion Exchange Protein 1, Erythrocyte
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Membrane Proteins
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Myelin Basic Protein
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Peptide Fragments
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Receptors, Antigen, T-Cell
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Recombinant Proteins
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antigen T cell receptor, zeta chain
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Protein-Tyrosine Kinases
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Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
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ZAP-70 Protein-Tyrosine Kinase
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ZAP70 protein, human
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src-Family Kinases