Functional and possible physical association of scavenger receptor with cytoplasmic tyrosine kinase Lyn in monocytic THP-1-derived macrophages

FEBS Lett. 1996 Dec 16;399(3):241-4. doi: 10.1016/s0014-5793(96)01332-4.

Abstract

Acetyl LDL (modified low-density lipoprotein), which is thought to be taken up through scavenger receptor A (SR-A), rapidly induced the appearance of phosphotyrosine proteins in monocytic THP-1-derived macrophages in vitro. The two alternative forms of Lyn (p53 and p56) were found to be tyrosine-phosphorylated within 30 s after the stimulation with acetyl LDL. The catalytic activity of Lyn measured by an in vitro kinase assay had also increased in acetyl LDL-stimulated THP-1-derived macrophages. Furthermore, Lyn could be co-immunoprecipitated with SR-A from the cell lysate. These observations suggest a functional and possible physical association of SR-A with Lyn in THP-1-derived macrophages, and also imply a possible involvement of Lyn in SR-A signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Cytosol / enzymology*
  • Foam Cells / metabolism*
  • Lipoproteins, LDL / chemistry
  • Lipoproteins, LDL / metabolism*
  • Membrane Proteins*
  • Molecular Sequence Data
  • Phosphorylation
  • Precipitin Tests
  • Receptors, Immunologic / chemistry
  • Receptors, Immunologic / metabolism*
  • Receptors, Lipoprotein*
  • Receptors, Scavenger
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • src-Family Kinases / metabolism*

Substances

  • Lipoproteins, LDL
  • Membrane Proteins
  • Receptors, Immunologic
  • Receptors, Lipoprotein
  • Receptors, Scavenger
  • Scarb1 protein, mouse
  • Scavenger Receptors, Class A
  • Scavenger Receptors, Class B
  • acetyl-LDL
  • lyn protein-tyrosine kinase
  • src-Family Kinases