Abstract
To examine the genetic and antigenic characteristics of HIV-1 in Indonesia, samples from 19 HIV-positive volunteers were studied. By a combination of PCR typing and DNA sequence analysis, 12 of the 19 volunteers were determined to be infected with HIV-1 clade B and seven with clade E. Six of the seven Indonesian clade E isolates were from volunteers associated with the Indonesian Military during a peacekeeping mission in Cambodia. Infectivity reduction neutralization assays showed that the Indonesian E viruses were effectively neutralized by Thailand clade E HIV-1 antisera but not by U.S. clade B antisera. The Indonesian clade B virus tested was neutralized by U.S. clade B antisera and not by the Thailand E antisera. Using a previously described serologic typing ELISA based on clade B and E V3 peptides, genetic clade was accurately determined in eight of eight sera tested. This is the first report of the genetic and antigenic analysis of HIV-1 isolates from Indonesia. The data indicate that at least two genetic and antigenic HIV-1 clades (clade E and B) circulate in Indonesia.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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CD4 Antigens / metabolism
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DNA, Viral / analysis
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DNA, Viral / chemistry
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Female
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Genotype
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HIV Antigens / immunology
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HIV Envelope Protein gp120 / chemistry
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / immunology
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HIV Seropositivity / epidemiology
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HIV Seropositivity / virology*
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HIV-1 / classification*
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HIV-1 / genetics
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HIV-1 / immunology
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Humans
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Immune Sera / immunology
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Indonesia / epidemiology
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Male
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Military Personnel
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Molecular Sequence Data
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Neutralization Tests
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Phylogeny
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Polymerase Chain Reaction
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Risk Factors
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Serotyping
Substances
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CD4 Antigens
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DNA, Viral
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HIV Antigens
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HIV Envelope Protein gp120
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HIV envelope protein gp120 (305-321)
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Immune Sera
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Peptide Fragments
Associated data
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GENBANK/U68188
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GENBANK/U68189
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GENBANK/U68190
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GENBANK/U68191
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GENBANK/U68192
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GENBANK/U68193
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GENBANK/U68194
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GENBANK/U68195
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GENBANK/U68196
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GENBANK/U68197
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GENBANK/U68198
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GENBANK/U68199
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GENBANK/U68200
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GENBANK/U68201