Long-term 1-carnitine treatment prolongs the survival in rats with adriamycin-induced heart failure

J Card Fail. 1996 Dec;2(4):293-9. doi: 10.1016/s1071-9164(96)80016-9.

Abstract

Background: The most serious consequence of heart failure is the shortened life expectancy, which may be associated with myocardial energy starvation.

Methods and results: Eight-week-old male Sprague-Dawley rats received 6 intraperitoneal injections of adriamycin (group A: total dose; 15 mg/kg body weight) or vehicle (group C) over 2 weeks. Rats then received either 272 mg/kg daily of oral 1-carnitine (A-LC and C-LC groups) or saline (A-S and C-S groups) for 6 weeks. The cumulative mortality rate in the A-LC group was significantly lower than in the A-S group (13 vs 42%, P = .028). Myocardial levels of high-energy phosphate compounds (ATP and creatine phosphate) and fatty acid metabolites (free carnitine, short-chain and long-chain acylcarnitine, and long-chain acyl CoA) in the left ventricle were measured the day after the last dose of drug or vehicle was administered. ATP was decreased by 73%, creatine phosphate by 61%, free carnitine by 52%, short-chain acylcarnitine by 48%, and long-chain acylcarnitine by 56% in the A-S group compared to the C-S group. Long-chain CoA was increased by 168% in the A-S group. Levels of myocardial high-energy phosphate compounds and fatty acid metabolites were near normal in adriamycin- and 1-carnitine-treated rats.

Conclusions: Preservation of the myocardial level of carnitine by 1-carnitine treatment prolonged survival of rats with adriamycin-induced failure by improving the myocardial metabolism of fatty acids.

MeSH terms

  • Acyl Coenzyme A / analysis*
  • Adenosine Triphosphate / analysis*
  • Administration, Oral
  • Analysis of Variance
  • Animals
  • Carnitine / administration & dosage
  • Carnitine / pharmacokinetics
  • Carnitine / therapeutic use*
  • Culture Techniques
  • Disease Models, Animal
  • Doxorubicin / pharmacology
  • Drug Administration Schedule
  • Heart Failure / chemically induced
  • Heart Failure / drug therapy*
  • Male
  • Myocardium / metabolism*
  • Myocardium / pathology
  • Phosphocreatine / analysis*
  • Rats
  • Rats, Sprague-Dawley
  • Survival Rate

Substances

  • Acyl Coenzyme A
  • Phosphocreatine
  • Doxorubicin
  • Adenosine Triphosphate
  • Carnitine