Abstract
The mRNA differential display technique was employed in order to identify novel growth-related genes linked to the mitogenic effect of PACAP on AR4-2J cells. Hereby, three differentially expressed and PACAP-inducible genes were found, one of these being homologous to a recently discovered putative growth-related early response gene in mice. In AR4-2J cells, mRNA levels of this novel rat gene are increased by PACAP in a rapid and transient fashion. This effect was enhanced by cycloheximide, whereas actinomycin D prevented the stimulatory effect of PACAP.
MeSH terms
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Animals
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Blotting, Northern
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Cell Division / drug effects*
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Cell Line
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Cycloheximide / pharmacology
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DNA Primers
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DNA, Complementary
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Dactinomycin / pharmacology
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Mice
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Neuropeptides / pharmacology*
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Neurotransmitter Agents / pharmacology
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Pancreas
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Pituitary Adenylate Cyclase-Activating Polypeptide
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RNA, Messenger / biosynthesis*
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Rats
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Transcription, Genetic / drug effects*
Substances
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Adcyap1 protein, mouse
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Adcyap1 protein, rat
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DNA Primers
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DNA, Complementary
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Neuropeptides
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Neurotransmitter Agents
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Pituitary Adenylate Cyclase-Activating Polypeptide
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RNA, Messenger
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Dactinomycin
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Cycloheximide