Identification of Rox3 as a component of mediator and RNA polymerase II holoenzyme

J Biol Chem. 1997 Jan 3;272(1):48-50. doi: 10.1074/jbc.272.1.48.

Abstract

Yeast Rox3 protein, implicated by genetic evidence in both negative and positive transcriptional regulation, is identified as a mediator subunit by peptide sequence determination and is shown to copurify and co-immunoprecipitate with RNA polymerase II holoenzyme.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Fungal Proteins / chemistry*
  • Gene Expression Regulation, Fungal
  • Macromolecular Substances
  • Mediator Complex
  • Molecular Sequence Data
  • Precipitin Tests
  • RNA Polymerase II / chemistry*
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / chemistry*

Substances

  • Fungal Proteins
  • Macromolecular Substances
  • Mediator Complex
  • ROX3 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RNA Polymerase II