Abstract
The interleukin-1beta (IL-1beta) converting enzyme (ICE) processes the inactive IL-1beta precursor to the proinflammatory cytokine. ICE was also shown to cleave the precursor of interferon-gamma inducing factor (IGIF) at the authentic processing site with high efficiency, thereby activating IGIF and facilitating its export. Lipopolysaccharide-activated ICE-deficient (ICE-/-) Kupffer cells synthesized the IGIF precursor but failed to process it into the active form. Interferon-gamma and IGIF were diminished in the sera of ICE-/- mice exposed to Propionibacterium acnes and lipopolysaccharide. The lack of multiple proinflammatory cytokines in ICE-/- mice may account for their protection from septic shock.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Caspase 1
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Caspase 3
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Caspases*
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Caspases, Initiator
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Culture Media, Conditioned
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Cysteine Endopeptidases / metabolism*
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Cytokines / blood
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Cytokines / metabolism*
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Cytokines / pharmacology
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Humans
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Interferon-gamma / biosynthesis
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Interferon-gamma / blood
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Interleukin-18
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Kupffer Cells / metabolism*
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Lipopolysaccharides / pharmacology
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Mice
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Protein Precursors / metabolism
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Protein Processing, Post-Translational
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Recombinant Proteins / metabolism
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Recombinant Proteins / pharmacology
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Spleen / cytology
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Spleen / metabolism
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Transfection
Substances
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Culture Media, Conditioned
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Cytokines
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Interleukin-18
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Lipopolysaccharides
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Protein Precursors
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Recombinant Proteins
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Interferon-gamma
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CASP3 protein, human
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CASP4 protein, human
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Casp3 protein, mouse
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Caspase 3
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Caspases
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Caspases, Initiator
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Cysteine Endopeptidases
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Caspase 1