Abstract
X-ray treatment induces a complex molecular response in hematopoietic cells leading to cell death. Using the mRNA differential display technique, we searched for genes whose expression was modified by ionizing radiation (IR) in the human p53-deficient leukemic cell line KG1a. We isolated a partial cDNA corresponding to the interferon (IFN)-inducible 1-8d gene. The expression of both 1-8d and 9-27, another gene from the same IFN-inducible family, was increased 24 and 48 h following irradiation. We did not find enhancement of either IFNgamma mRNA or interferon regulatory factor-1 (IRF-1) mRNA in irradiated KG1a cells, indicating that 1-8d and 9-27 enhancement was not due to an IFN activation. Thus, the induction of IFN-inducible genes by IR may provide a link between radiation-induced and IFN-mediated cell death.
MeSH terms
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Base Sequence
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Cell Death / genetics*
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Cell Line / radiation effects
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DNA, Complementary / isolation & purification*
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DNA-Binding Proteins / analysis
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Gene Amplification / drug effects
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Gene Amplification / genetics
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Gene Amplification / radiation effects
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Gene Expression Regulation / drug effects*
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Gene Expression Regulation / genetics
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Gene Expression Regulation / radiation effects*
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Genes, Immediate-Early / drug effects*
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Genes, Immediate-Early / genetics
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Genes, Immediate-Early / radiation effects*
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Genes, cdc / drug effects*
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Genes, cdc / genetics
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Genes, cdc / radiation effects*
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Humans
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Interferon Regulatory Factor-1
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Interferon-gamma / pharmacology*
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Molecular Sequence Data
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Phosphoproteins / analysis
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RNA, Messenger / metabolism
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RNA, Messenger / radiation effects
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Sequence Analysis, DNA
Substances
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DNA, Complementary
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DNA-Binding Proteins
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IRF1 protein, human
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Interferon Regulatory Factor-1
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Phosphoproteins
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RNA, Messenger
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Interferon-gamma