Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System

G Boivin; J Handfield; G Murray; E Toma; R Lalonde; J G Lazar; M G Bergeron

doi:10.1128/jcm.35.2.525-526.1997

Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP Signal Detection System

J Clin Microbiol. 1997 Feb;35(2):525-6. doi: 10.1128/jcm.35.2.525-526.1997.

Authors

G Boivin¹, J Handfield, G Murray, E Toma, R Lalonde, J G Lazar, M G Bergeron

Affiliation

¹ Department of Microbiology, Université Laval, Quebec City, Canada.

Abstract

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

AIDS-Related Opportunistic Infections / virology*
Antigens, Viral / blood
Cytomegalovirus / genetics
Cytomegalovirus / isolation & purification*
Cytomegalovirus Infections / virology*
DNA, Viral / blood*
HIV Infections / virology
Humans
Neutrophils / virology*
Polymerase Chain Reaction / methods*
Reproducibility of Results
Sensitivity and Specificity

Substances

Antigens, Viral
DNA, Viral