Inflammation-seeking scintigraphy with radiolabeled biotinylated polyclonal IgG followed by the injection of avidin chase

Nucl Med Biol. 1996 Jan;23(1):29-32. doi: 10.1016/0969-8051(95)02010-1.

Abstract

We tried to apply the avidin chasing system to the inflammation-seeking scintigraphy using radiolabeled nonspecific polyclonal IgG. We studied the pharmacokinetics of technetium-99m and iodine-125-labeled biotinylated murine polyclonal IgG followed by an avidin chase injection in model mice with inflammatory foci. Avidin chase decreased the circulating radioactivity of 99mTc and 125I, which was a major problem for inflammation-seeking scintigraphy using radiolabeled nonspecific polyclonal IgG, to 9.3% and 19.3% of that without an avidin chase injection, respectively. Inflammation-seeking scintigraphy with the aforementioned method would be better than that with conventional method.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacokinetics
  • Avidin*
  • Biotin* / pharmacokinetics
  • Immunoconjugates* / pharmacokinetics
  • Immunoglobulin G*
  • Inflammation / diagnostic imaging*
  • Iodine Radioisotopes
  • Isotope Labeling
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Quality Control
  • Radionuclide Imaging
  • Technetium
  • Tissue Distribution

Substances

  • Antibodies, Monoclonal
  • Immunoconjugates
  • Immunoglobulin G
  • Iodine Radioisotopes
  • Avidin
  • Biotin
  • Technetium