Abstract
Mutations in the Caenorhabditis elegans gene clk-1 affect biological timing and extend longevity. The gene clk-1 was identified, and the cloned gene complemented the clk-1 phenotypes and restored normal longevity. The CLK-1 protein was found to be conserved among eukaryotes, including humans, and structurally similar to the yeast metabolic regulator Cat5p (also called Coq7p). These proteins contain a tandem duplication of a core 82-residue domain. clk-1 complemented the phenotype of cat5/coq7 null mutants, demonstrating that clk-1 and CAT5/COQ7 share biochemical function and that clk-1 acts at the level of cellular physiology.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / metabolism
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins*
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Cellular Senescence / genetics*
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Chromosome Mapping
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Conserved Sequence
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Exons
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / physiology
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Genes, Helminth*
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Genetic Complementation Test
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Glycerol / metabolism
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Helminth Proteins / chemistry
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Helminth Proteins / genetics*
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Helminth Proteins / physiology
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Humans
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Longevity / genetics
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Mice
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Molecular Sequence Data
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Phenotype
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Protein Structure, Secondary
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Protein Structure, Tertiary
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RNA Splicing
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Saccharomyces cerevisiae Proteins*
Substances
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CAT5 protein, S cerevisiae
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CLK-1 protein, C elegans
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Caenorhabditis elegans Proteins
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Fungal Proteins
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Helminth Proteins
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Saccharomyces cerevisiae Proteins
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Glycerol
Associated data
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GENBANK/U81276
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GENBANK/U81277