Abstract
The L-carbamoylase gene (amaB) upstream of the previously detected L-aminoacylase gene (amaA) in the Bacillus stearothermophilus NCIB8224 strain was identified in this study. The amaB and amaA genes are cotranscribed as a single mRNA from the same transcriptional start. The two-ama-gene operon is conserved in B. stearothermophilus strains. A cross-activity of L-carbamoylase towards respective substrates for L-aminoacylase supports the hypothesis of a common ancestor for both amino acid amidohydrolase genes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amidohydrolases / genetics*
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Amidohydrolases / immunology
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Bacterial Proteins / genetics
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Base Sequence
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Cloning, Molecular
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Cross Reactions
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Genes, Bacterial*
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Geobacillus stearothermophilus / enzymology
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Geobacillus stearothermophilus / genetics*
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Molecular Sequence Data
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Operon*
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RNA, Bacterial / biosynthesis
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RNA, Messenger / biosynthesis
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Sequence Analysis, DNA
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Stereoisomerism
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Transcription, Genetic
Substances
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Bacterial Proteins
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RNA, Bacterial
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RNA, Messenger
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Amidohydrolases
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aminoacylase I
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N-carbamoyl-L-amino-acid hydrolase
Associated data
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GENBANK/Y08751
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GENBANK/Y08752
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GENBANK/Y08753
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GENBANK/Y08754