Expression and characterization of a mouse/human chimeric antibody specific for EGF receptor

C Ferrer; A M Anastasi; A M Di Massimo; A Bullo; M Di Loreto; G Raucci; A Pacilli; L Rotondaro; S Mauro; A Mele; R De Santis

doi:10.1016/s0168-1656(96)01625-2

Expression and characterization of a mouse/human chimeric antibody specific for EGF receptor

J Biotechnol. 1996 Nov 29;52(1):51-60. doi: 10.1016/s0168-1656(96)01625-2.

Authors

C Ferrer¹, A M Anastasi, A M Di Massimo, A Bullo, M Di Loreto, G Raucci, A Pacilli, L Rotondaro, S Mauro, A Mele, R De Santis

Affiliation

¹ Menarini Ricerche SpA, Department of Biotechnology, Pomezia, Rome, Italy.

PMID: 9025323
DOI: 10.1016/s0168-1656(96)01625-2

Abstract

Murine antibodies which recognize the epidermal growth factor receptor (EGF-r) are good candidates for therapy and diagnosis of tumors overexpressing this receptor. Here we report the isolation of the variable regions from a murine monoclonal antibody anti-EGF-r (Mint5), the procedure to obtain the mouse/human chimeric antibody (chMint5) and its expression in COS, NS0 and CHO cells. The approach followed to construct chMint5 is based on the use of consensus primers specific for the ends of the variable regions. The sequence imposed by the primers did not affect the targeting potential of the antibody. In fact, the affinity of the chimeric antibody for EGF-r was nearly the same as that of the parental murine antibody. Based on previous in vitro and in vivo animal studies. Mint5 was shown to be a good candidate for the targeting of EGF-r overexpressing tumours. chMint5 is expected to be less immunogenic than murine antibody and therefore, could be useful for human treatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibodies, Monoclonal / biosynthesis*
Antibodies, Monoclonal / genetics*
Antibody Affinity
Antibody Specificity
Base Sequence
COS Cells / immunology
COS Cells / metabolism
Carcinoma, Squamous Cell / immunology
Carcinoma, Squamous Cell / pathology
Epitopes
ErbB Receptors / immunology*
Genetic Vectors
Humans
Immunoglobulin Heavy Chains / genetics
Immunoglobulin Light Chains / genetics
Immunoglobulin Variable Region / genetics
Immunoglobulin gamma-Chains / genetics
Immunoglobulin kappa-Chains / genetics
Mice
Molecular Sequence Data
Multiple Myeloma / genetics
Multiple Myeloma / immunology
Multiple Myeloma / pathology
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / immunology*
Transfection
Tumor Cells, Cultured

Substances

Antibodies, Monoclonal
Epitopes
Immunoglobulin Heavy Chains
Immunoglobulin Light Chains
Immunoglobulin Variable Region
Immunoglobulin gamma-Chains
Immunoglobulin kappa-Chains
Recombinant Fusion Proteins
ErbB Receptors