Kinetic role of early intermediates in protein folding

Curr Opin Struct Biol. 1997 Feb;7(1):15-28. doi: 10.1016/s0959-440x(97)80004-8.

Abstract

The traditional view that partly folded intermediates are important for directing a protein toward the native state has been challenged by the notion that proteins can intrinsically fold rapidly in a single step if kinetic complications due to slow conformational events are avoided. Intermediates that accumulate within the first few milliseconds of folding are, however, a common observation even for small single-domain proteins. Recent spectroscopic studies, coupled with quantitative kinetic analysis, suggest that folding is facilitated by the rapid formation of compact intermediates with some native-like structural features.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Fluorescent Dyes
  • Kinetics
  • Mutagenesis, Site-Directed
  • Protein Folding*
  • Protein Structure, Secondary
  • Solvents

Substances

  • Fluorescent Dyes
  • Solvents