Human immunodeficiency virus strains differ in their ability to infect CD4+ cells expressing the rat homolog of CXCR-4 (fusin)

J Virol. 1997 Apr;71(4):3259-62. doi: 10.1128/JVI.71.4.3259-3262.1997.

Abstract

A clade B strain of human immunodeficiency virus type 1 (HIV-1(LAI)) could infect CD4+ cells expressing human CXCR-4 (fusin) or its rat homolog with similar efficacy. By contrast, cells expressing rat CXCR-4 were not permissive to HIV-1(NDK) (clade D), HIV-2(ROD), or HIV-1(LAI) with chimeric envelope protein gp120 bearing the V3 domain from HIV-1(NDK). The reciprocal chimeric gp120 (HIV-1(NDK) with V3 from HIV-1(LAI)) could mediate infection of cells expressing either human or rat CXCR-4. Genetically divergent HIV strains have different requirements for interaction with the CXCR-4 coreceptor, and the gp120 V3 domain seems to be involved in this interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / virology*
  • HIV Core Protein p24 / analysis
  • HIV Envelope Protein gp120 / genetics
  • HIV Envelope Protein gp120 / immunology
  • HIV-1 / classification
  • HIV-1 / genetics
  • HIV-1 / physiology*
  • HIV-2 / classification
  • HIV-2 / physiology*
  • HeLa Cells
  • Humans
  • Membrane Proteins / biosynthesis
  • Membrane Proteins / genetics
  • Membrane Proteins / physiology*
  • Peptide Fragments / genetics
  • Peptide Fragments / immunology
  • Rats
  • Receptors, CXCR4
  • Receptors, HIV / biosynthesis
  • Receptors, HIV / genetics
  • Receptors, HIV / physiology*
  • Recombinant Fusion Proteins / genetics
  • Species Specificity
  • Tumor Cells, Cultured

Substances

  • HIV Core Protein p24
  • HIV Envelope Protein gp120
  • HIV envelope protein gp120 (305-321)
  • Membrane Proteins
  • Peptide Fragments
  • Receptors, CXCR4
  • Receptors, HIV
  • Recombinant Fusion Proteins