Abstract
A clade B strain of human immunodeficiency virus type 1 (HIV-1(LAI)) could infect CD4+ cells expressing human CXCR-4 (fusin) or its rat homolog with similar efficacy. By contrast, cells expressing rat CXCR-4 were not permissive to HIV-1(NDK) (clade D), HIV-2(ROD), or HIV-1(LAI) with chimeric envelope protein gp120 bearing the V3 domain from HIV-1(NDK). The reciprocal chimeric gp120 (HIV-1(NDK) with V3 from HIV-1(LAI)) could mediate infection of cells expressing either human or rat CXCR-4. Genetically divergent HIV strains have different requirements for interaction with the CXCR-4 coreceptor, and the gp120 V3 domain seems to be involved in this interaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / metabolism
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CD4-Positive T-Lymphocytes / virology*
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HIV Core Protein p24 / analysis
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HIV Envelope Protein gp120 / genetics
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HIV Envelope Protein gp120 / immunology
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HIV-1 / classification
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HIV-1 / genetics
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HIV-1 / physiology*
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HIV-2 / classification
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HIV-2 / physiology*
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HeLa Cells
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Humans
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Membrane Proteins / physiology*
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Peptide Fragments / genetics
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Peptide Fragments / immunology
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Rats
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Receptors, CXCR4
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Receptors, HIV / biosynthesis
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Receptors, HIV / genetics
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Receptors, HIV / physiology*
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Recombinant Fusion Proteins / genetics
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Species Specificity
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Tumor Cells, Cultured
Substances
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HIV Core Protein p24
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HIV Envelope Protein gp120
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HIV envelope protein gp120 (305-321)
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Membrane Proteins
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Peptide Fragments
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Receptors, CXCR4
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Receptors, HIV
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Recombinant Fusion Proteins