Purpose: Large-cell lymphoma can be difficult to diagnose because vitreous biopsies may fail to disclose neoplastic cells.
Methods: We report one such case in which diagnosis was confirmed by subretinal aspiration of yellow-white infiltrates using a pars plana approach.
Results: A 67-year-old woman with recurrent bilateral uveitis was diagnosed with large-cell lymphoma by subretinal aspiration of yellow-white infiltrates after two vitreous biopsies and a concurrent retinal biopsy failed to confirm the diagnosis.
Conclusions: When the suspicion of intraocular lymphoma remains high despite previous negative vitreous biopsies, retinal biopsy and aspiration biopsy of subretinal lesions may enhance the diagnostic yield.