Scanning mutagenesis to localize DNA-protein interactions

Biotechniques. 1997 Mar;22(3):434-6. doi: 10.2144/97223bm12.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Binding Sites
  • CD18 Antigens / chemistry
  • CD18 Antigens / genetics*
  • DNA / metabolism*
  • DNA Primers / chemistry
  • DNA-Binding Proteins / metabolism*
  • Deoxyribonuclease HindIII / metabolism
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Leukocytes / metabolism
  • Molecular Sequence Data
  • Mutagenesis*
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • Proto-Oncogene Proteins / metabolism*
  • Trans-Activators / metabolism*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic / genetics

Substances

  • CD18 Antigens
  • DNA Primers
  • DNA-Binding Proteins
  • Proto-Oncogene Proteins
  • Trans-Activators
  • Transcription Factors
  • proto-oncogene protein Spi-1
  • DNA
  • Deoxyribonuclease HindIII
  • endodeoxyribonuclease SacI
  • Deoxyribonucleases, Type II Site-Specific