Abstract
NURR1 is an immediate early gene product and a member of the nuclear receptor superfamily of transcription factors. Using the NURR1 cDNA as a probe, we isolated the genomic DNA encoding NURR1 from a mouse 129SvEv genomic library. The NURR1 gene is approximately 6.2 kb long and is organized into 7 exons separated by 6 introns. Structural analysis of the NURR1 reveals that this gene shares a similar structure with that of the nuclear receptor NUR77/NGF1-B. As in NUR77, the promoter region of NURR1 lacks an identifiable TATA box, but is GC-rich. The proximal promoter region also contains an ATF/CREB consensus binding site that may participate in cAMP-mediated induction of this immediate early gene product. Isolation and structural characterization of the NURR1 gene provides information for further developmental and transcriptional regulation studies of this gene.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Activating Transcription Factor 1
-
Animals
-
Base Sequence
-
Binding Sites
-
Cloning, Molecular
-
Consensus Sequence
-
Cyclic AMP Response Element-Binding Protein / metabolism
-
DNA-Binding Proteins / chemistry
-
DNA-Binding Proteins / genetics*
-
Exons / genetics
-
Introns / genetics
-
Mice
-
Molecular Sequence Data
-
Nerve Tissue Proteins / chemistry
-
Nerve Tissue Proteins / genetics*
-
Nuclear Receptor Subfamily 4, Group A, Member 1
-
Nuclear Receptor Subfamily 4, Group A, Member 2
-
Promoter Regions, Genetic
-
Receptors, Cytoplasmic and Nuclear
-
Receptors, Steroid
-
Transcription Factors / chemistry
-
Transcription Factors / genetics*
-
Transcription Factors / metabolism
-
Transcription, Genetic
Substances
-
Activating Transcription Factor 1
-
Cyclic AMP Response Element-Binding Protein
-
DNA-Binding Proteins
-
Nerve Tissue Proteins
-
Nr4a1 protein, mouse
-
Nr4a2 protein, mouse
-
Nuclear Receptor Subfamily 4, Group A, Member 1
-
Nuclear Receptor Subfamily 4, Group A, Member 2
-
Receptors, Cytoplasmic and Nuclear
-
Receptors, Steroid
-
Transcription Factors