Abstract
Mice whose IgH alleles are engineered to encode two distinct antibody heavy (H) chains generate a normal-sized B cell compartment in which most cells stably express the two heavy chains. This demonstrates that "toxicity" of bi-allelic H chain expression and cell-autonomous mechanisms of silencing in-frame IgH gene rearrangements do not significantly contribute to allelic exclusion at the IgH locus. Notwithstanding, the stability of the various engineered IgH loci during B cell development in the bone marrow differed substantially from each other.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alleles*
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Animals
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B-Lymphocytes / cytology*
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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Cell Compartmentation / immunology
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Frameshift Mutation / immunology
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Gene Rearrangement, B-Lymphocyte, Heavy Chain
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Immunoglobulin Joining Region / genetics
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Immunoglobulin Variable Region / genetics
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Mice
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Mice, Mutant Strains
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Molecular Sequence Data
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Polymorphism, Genetic / immunology
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Receptors, Antigen, B-Cell / metabolism
Substances
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Immunoglobulin Joining Region
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Immunoglobulin Variable Region
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Receptors, Antigen, B-Cell
Associated data
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GENBANK/Z84197
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GENBANK/Z84198
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GENBANK/Z84199
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GENBANK/Z84200