Abstract
The proposed cis-Golgi vesicle receptor syntaxin 5 was found in a complex with Golgi-associated SNARE of 28 kDa (GOS-28), rbet1, rsly1, and two novel proteins characterized herein: rat sec22b and membrin, both cytoplasmically oriented integral membrane proteins. The complex appears to recapitulate vesicle docking interactions of proteins originating from distinct compartments, since syntaxin 5, rbet1, and GOS-28 localize to Golgi membranes, whereas mouse sec22b and membrin accumulate in the endoplasmic reticulum. Protein interactions in the complex are dramatically rearranged by N-ethylmaleimide-sensitive factor. The complex consists of two or more subcomplexes with some members (rat sec22b and syntaxin 5) in common and others (rbet1 and GOS-28) mutually exclusively associated. We propose that these protein interactions determine vesicle docking/fusion fidelity between the endoplasmic reticulum and Golgi.
MeSH terms
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Animals
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Biological Transport / physiology
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COS Cells / physiology
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Carrier Proteins / physiology
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Detergents
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Endoplasmic Reticulum / chemistry
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Endoplasmic Reticulum / metabolism*
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Epitopes / analysis
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Gene Expression / physiology
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Golgi Apparatus / chemistry
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Golgi Apparatus / metabolism*
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Mammals
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Membrane Proteins / analysis
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Membrane Proteins / genetics*
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Membrane Proteins / isolation & purification
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Membrane Proteins / physiology
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Mice
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Molecular Sequence Data
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N-Ethylmaleimide-Sensitive Proteins
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / isolation & purification
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Neurons / cytology
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Neurons / metabolism
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Qa-SNARE Proteins
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Qb-SNARE Proteins
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Qc-SNARE Proteins
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R-SNARE Proteins
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Rabbits
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Rats
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Rats, Sprague-Dawley
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Sequence Homology, Amino Acid
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Subcellular Fractions / chemistry
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Synaptic Vesicles / chemistry
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Synaptic Vesicles / metabolism*
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Vesicular Transport Proteins*
Substances
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BET1 protein, rat
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Carrier Proteins
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Detergents
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Epitopes
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Gosr1 protein, mouse
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Gosr1 protein, rat
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Gosr2 protein, mouse
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Gosr2 protein, rat
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Membrane Proteins
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Nerve Tissue Proteins
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Qa-SNARE Proteins
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Qb-SNARE Proteins
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Qc-SNARE Proteins
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R-SNARE Proteins
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Sec22a protein, rat
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins
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N-Ethylmaleimide-Sensitive Proteins
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Nsf protein, mouse
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Nsf protein, rat
Associated data
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GENBANK/U91538
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GENBANK/U91539