Immunoassay reagents for thyroid testing. 2. Binding properties and energetic parameters of a T4 monoclonal antibody and its Fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance

M Adamczyk; J C Gebler; A H Gunasekera; P G Mattingly; Y Pan

doi:10.1021/bc960088z

Immunoassay reagents for thyroid testing. 2. Binding properties and energetic parameters of a T4 monoclonal antibody and its Fab fragment with a library of thyroxine analog biosensors using surface plasmon resonance

Bioconjug Chem. 1997 Mar-Apr;8(2):133-45. doi: 10.1021/bc960088z.

Authors

M Adamczyk¹, J C Gebler, A H Gunasekera, P G Mattingly, Y Pan

Affiliation

¹ Division Organic Chemistry (9-NM), Abbott Laboratories, Abbott Park, Illinois 60064, USA. [email protected]

PMID: 9095353
DOI: 10.1021/bc960088z

Abstract

A library of 16 thyroxine analog biosensors were prepared for use on the BIAcore surface plasmon resonance instrument. An anti-thyroxine monoclonal antibody and its Fab fragment were tested with each biosensor to generate the association and dissociation rate constants, equilibrium association constants, and Gibbs free energy values for characterizing the binding event.

MeSH terms

Animals
Antibodies, Monoclonal
Binding Sites
Biosensing Techniques*
Electrophoresis, Polyacrylamide Gel
Humans
Immunoassay / instrumentation
Immunoassay / methods*
Immunoglobulin Fab Fragments
Indicators and Reagents
Kinetics
Mass Spectrometry
Molecular Structure
Thermodynamics
Thyroid Function Tests / methods*
Thyroxine / analogs & derivatives*
Thyroxine / chemistry
Thyroxine / immunology*

Substances

Antibodies, Monoclonal
Immunoglobulin Fab Fragments
Indicators and Reagents
Thyroxine