An outer surface of a 4 mm polytetrafluoroethylene (PTFE) prosthesis, Gore-Tex thin-walled stretch graft, was treated by the fibrin glue spray with a 1.0 kg/m2 compressed air. The distance between the PTFE graft and a spray-nozzle was within 0.5 cm. After the treatment, an outer surface and a cross-section of the PTFE graft were examined under a scanning electron microscope. Fibrin glue mended the gap of the PTFE fibril of an outer surface and a fibrin glue layer adhered firmly to the graft. The fibrin glue spray treatment may prevent postoperative serous fluid leakage.