Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast

I Gruic-Sovulj; H C Lüdemann; F Hillenkamp; I Weygand-Durasevic; Z Kucan; J Peter-Katalinic

doi:10.1093/nar/25.9.1859

Matrix-assisted laser desorption/ionisation mass spectrometry of transfer ribonucleic acids isolated from yeast

Nucleic Acids Res. 1997 May 1;25(9):1859-61. doi: 10.1093/nar/25.9.1859.

Authors

I Gruic-Sovulj¹, H C Lüdemann, F Hillenkamp, I Weygand-Durasevic, Z Kucan, J Peter-Katalinic

Affiliation

¹ Department of Biochemistry, Faculty of Science, University of Zagreb, Strossmayerov trg 14, 10000 Zagreb, Croatia.

Abstract

tRNATyr and tRNASer purified from bulk brewer's yeast tRNA were subjected to analysis by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Choosing a mixture of 2,4,6- and 2,3,4-trihydroxy-acetophenone and diammonium citrate as matrix a mass resolution of up to 220 (FWHM) was achieved in the linear mode of operation. Cation adduct suppression by addition of cation exchange beads and a chelating agent (CDTA) is shown to substantially improve mass resolution for this class of molecules.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

RNA, Fungal / chemistry*
RNA, Transfer / chemistry*
Saccharomyces cerevisiae / genetics*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

RNA, Fungal
RNA, Transfer