Abstract
The neurofibromatosis type 1 (NF1) tumor suppressor protein is thought to restrict cell proliferation by functioning as a Ras-specific guanosine triphosphatase-activating protein. However, Drosophila homozygous for null mutations of an NF1 homolog showed no obvious signs of perturbed Ras1-mediated signaling. Loss of NF1 resulted in a reduction in size of larvae, pupae, and adults. This size defect was not modified by manipulating Ras1 signaling but was restored by expression of activated adenosine 3', 5'-monophosphate-dependent protein kinase (PKA). Thus, NF1 and PKA appear to interact in a pathway that controls the overall growth of Drosophila.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Count
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Cyclic AMP / metabolism
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Cyclic AMP-Dependent Protein Kinases / genetics
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Cyclic AMP-Dependent Protein Kinases / metabolism*
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Drosophila / cytology
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Drosophila / genetics*
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Drosophila / growth & development
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Drosophila / metabolism
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Drosophila Proteins*
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GTP Phosphohydrolases / metabolism
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Genes, Insect
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Insect Proteins / chemistry
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Insect Proteins / genetics
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Insect Proteins / metabolism*
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Molecular Sequence Data
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Mutation
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Nerve Tissue Proteins*
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Neurofibromin 1
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Phenotype
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Proteins / chemistry
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Proteins / genetics
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Recombinant Fusion Proteins / pharmacology
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Signal Transduction
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ras GTPase-Activating Proteins*
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ras Proteins / metabolism
Substances
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Drosophila Proteins
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Insect Proteins
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NF1 protein, Drosophila
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Nerve Tissue Proteins
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Neurofibromin 1
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Proteins
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Recombinant Fusion Proteins
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ras GTPase-Activating Proteins
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Cyclic AMP
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Cyclic AMP-Dependent Protein Kinases
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GTP Phosphohydrolases
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ras Proteins
Associated data
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GENBANK/L26500
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GENBANK/L26501
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GENBANK/L26502