Siderophore production by Staphylococcus aureus and identification of iron-regulated proteins

Infect Immun. 1997 May;65(5):1944-8. doi: 10.1128/iai.65.5.1944-1948.1997.

Abstract

Siderophore activity of Staphylococcus aureus was detected in an iron-restricted chemically defined medium. The molecular mass of this siderophore, called aureochelin, was 577 Da. Surface-associated proteins of 120, 88, 57, 35, and 33 kDa were mainly expressed under iron restriction conditions. Results showed a relationship between siderophore production and the existence of the 120- and 88-kDa proteins. Western blotting of surface-associated proteins revealed that these proteins were recognized both by patients sera and polyclonal rabbit serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / analysis
  • Bacterial Proteins / immunology
  • Bacterial Proteins / metabolism
  • Blotting, Western
  • Colony Count, Microbial
  • Culture Media / metabolism
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Iron / metabolism
  • Kinetics
  • Mass Spectrometry
  • Siderophores / metabolism*
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / metabolism*

Substances

  • Bacterial Proteins
  • Culture Media
  • Siderophores
  • Iron