High-level expression of cecropin CMIV in E. coli from a fusion construct containing the human tumor necrosis factor

L Wang; H Wu; F Dou; W Xie; X Xu

doi:10.1080/15216549700202121

High-level expression of cecropin CMIV in E. coli from a fusion construct containing the human tumor necrosis factor

Biochem Mol Biol Int. 1997 Apr;41(5):1051-6. doi: 10.1080/15216549700202121.

Authors

L Wang¹, H Wu, F Dou, W Xie, X Xu

Affiliation

¹ Department of Biochemistry, Nanjing University, P.R. China.

PMID: 9137837
DOI: 10.1080/15216549700202121

Abstract

Cecropin CMIV gene was fused to the 3'-terminus of the mutated tumor necrosis factor (TNFb) gene and the fusion gene was directly under the control of an inducible T7 promoter in pET-11d. This fusion gene was overexpressed in Escherichia coli with an expression level of approximate 40%-50% of total cellular proteins, and was produced mainly in the form of inclusion body. Peptide with antibacterial activity was obtained by cleaving the fusion protein with CNBr.

MeSH terms

Escherichia coli / genetics
Escherichia coli / metabolism*
Humans
Insect Hormones / biosynthesis*
Insect Hormones / genetics*
Insect Hormones / metabolism
Plasmids / genetics
Recombinant Fusion Proteins / biosynthesis*
Recombinant Fusion Proteins / genetics*
Recombinant Fusion Proteins / metabolism
Tumor Necrosis Factor-alpha / biosynthesis
Tumor Necrosis Factor-alpha / genetics*

Substances

Insect Hormones
Recombinant Fusion Proteins
Tumor Necrosis Factor-alpha