Two experiments were designed to determine the tissue distribution of threonine oxidation through the threonine dehydrogenase (EC 1.1.1.103) pathway in pigs. The first experiment was conducted on eleven Piétrain x Large White piglets. The piglets were slaughtered at 5, 12 or 20 kg after 1 h of infusion with L-[U-14C]threonine (55 kBq/kg) mixed with unlabelled threonine (100 mg/kg). In the second experiment, four Piétrain x Large White and four large White piglets (10 kg body weight) were infused with L-[1-13C]threonine (50 mg/kg) mixed with 50 mg/kg unlabelled threonine for 1 h, then killed for tissue sampling. In the two experiments, threonine dehydrogenase specific activity and threonine and glycine specific radioactivities and enrichments were measured in several tissues and in plasma. The higher level of labelling of threonine in the pancreas than in the liver suggested either a lower protein degradation rate or a faster rate of threonine transport in the liver than in the pancreas. Threonine dehydrogenase activity was found only in the liver and the pancreas. Whereas liver and pancreas threonine dehydrogenase specific activities were similar, glycine specific radioactivity and enrichment were 12- to 14-fold higher in the pancreas than in the liver. This is probably the consequence of a higher production rate of glycine from sources other than threonine (protein degradation, de novo synthesis from serine) in the liver than in the pancreas. Our results showed that Large White pigs could oxidize more threonine than Piétrain x Large White pigs. This could be related to the difference in growth performance and dietary N efficiency for protein deposition between these two genotypes.