Abstract
A new gene encoding a heat shock protein 70 family protein of Schizosaccharomyces pombe (Sp), named sks2+, was cloned as a weak suppressor for the K-252a-sensitive mutation, ucm1. The nucleotide sequence of sks2+ revealed an open reading frame of a 613-amino-acid (aa) protein. The deduced aa sequence of sks2+ showed significant homology with Saccharomyces cerevisiae (Sc) Ssb1p and Ssb2p responsible for protein synthesis by non-organelle-localized ribosomes, as well as with other proteins of the HSP70 family. The cells lacking the functional sks2+ gene were viable and showed no increased sensitivity to K-252a but grew slowly with an elongated morphology. These results suggest that the sks2+ gene product plays a role in the cell cycle progression and is able to confer drug resistance in a multicopy state.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carbazoles / pharmacology*
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Cell Cycle / genetics
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Cloning, Molecular
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Drug Resistance, Microbial
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Fungal Proteins / genetics
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Fungal Proteins / isolation & purification
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Fungal Proteins / physiology
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Genes, Fungal / physiology
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HSP70 Heat-Shock Proteins / genetics*
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HSP70 Heat-Shock Proteins / isolation & purification
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HSP70 Heat-Shock Proteins / physiology
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Indole Alkaloids
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Molecular Sequence Data
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Mutagenesis, Insertional
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Protein Kinase C / antagonists & inhibitors
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Schizosaccharomyces / cytology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / physiology
Substances
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Carbazoles
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Fungal Proteins
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HSP70 Heat-Shock Proteins
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Indole Alkaloids
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staurosporine aglycone
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Protein Kinase C
Associated data
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GENBANK/D63798
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GENBANK/D88479
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GENBANK/X13713