Abstract
The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins*
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Cell Membrane
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Cytochrome b Group / genetics
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Cytochrome b Group / metabolism*
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Electron Transport
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Enzyme Stability
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Genes, Bacterial / genetics
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Hydrogen / metabolism*
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Hydrogenase / analysis
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Hydrogenase / genetics
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Hydrogenase / metabolism
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Molecular Sequence Data
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Multigene Family / genetics
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Oxidation-Reduction
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Oxygen Consumption
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Pseudomonas / enzymology
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Pseudomonas / genetics
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Pseudomonas / growth & development
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Pseudomonas / metabolism*
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Restriction Mapping
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Cytochrome b Group
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hupC protein, Bacteria
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Hydrogen
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Hydrogenase
Associated data
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GENBANK/D76419
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GENBANK/D86986
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GENBANK/L23970
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GENBANK/M21679
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GENBANK/M60116
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GENBANK/M96433