Abstract
We investigated gelatinolytic enzymes derived from cultured human keratinocytes. Using a zymograph, we detected 92 kDa and 72 kDa gelatinases as major components and 83 kDa as a minor component from a conditioned cultured medium (BSL-K110, Kyokutoseiyaku Co.) of keratinocytes. After several passages, the 72 kDa band disappeared, and the 83 kDa band became dominant in another conditioned culture medium (K-GM, Kurashikibouseki Co.); these keratinocytes had a relatively differentiated appearance. These results suggest that some mechanism may regulate the secretion of selected types of gelatinase from keratinocytes under different conditions.
MeSH terms
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Cell Differentiation
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Cells, Cultured
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Collagenases / classification
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Collagenases / metabolism
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Coloring Agents
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Culture Media
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Culture Media, Conditioned
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Electrophoresis, Polyacrylamide Gel
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Gelatinases / classification
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Gelatinases / metabolism*
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Humans
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Keratinocytes / cytology
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Keratinocytes / enzymology*
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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Metalloendopeptidases / classification
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Metalloendopeptidases / metabolism
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Phenylmercuric Acetate / analogs & derivatives
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Sodium Dodecyl Sulfate
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Sulfhydryl Reagents
Substances
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Coloring Agents
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Culture Media
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Culture Media, Conditioned
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Sulfhydryl Reagents
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Sodium Dodecyl Sulfate
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4-aminophenylmercuriacetate
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Collagenases
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Gelatinases
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Metalloendopeptidases
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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Phenylmercuric Acetate