Abstract
We report the identification and characterization of ERS-24 (Endoplasmic Reticulum SNARE of 24 kD), a new mammalian v-SNARE implicated in vesicular transport between the ER and the Golgi. ERS24 is incorporated into 20S docking and fusion particles and disassembles from this complex in an ATP-dependent manner. ERS-24 has significant sequence homology to Sec22p, a v-SNARE in Saccharomyces cerevisiae required for transport between the ER and the Golgi. ERS-24 is localized to the ER and to the Golgi, and it is enriched in transport vesicles associated with these organelles.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / metabolism
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Animals
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Base Sequence
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Biological Transport / physiology
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CHO Cells / chemistry
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CHO Cells / physiology
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Carrier Proteins / genetics*
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Carrier Proteins / isolation & purification
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Carrier Proteins / metabolism
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Cell Membrane / chemistry
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Cell Membrane / enzymology
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Cloning, Molecular
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Cricetinae
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Endoplasmic Reticulum / chemistry*
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Endoplasmic Reticulum / metabolism*
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Endoplasmic Reticulum / ultrastructure
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Fungal Proteins / genetics
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Golgi Apparatus / chemistry*
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Leukemia, Basophilic, Acute
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Mammals
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Membrane Proteins / genetics*
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Membrane Proteins / isolation & purification
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Membrane Proteins / metabolism
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Microscopy, Immunoelectron
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Molecular Sequence Data
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Nerve Tissue Proteins / metabolism
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Qa-SNARE Proteins
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R-SNARE Proteins
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Rats
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SNARE Proteins
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Sequence Homology, Amino Acid
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Tumor Cells, Cultured
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Vesicular Transport Proteins*
Substances
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Carrier Proteins
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ERS-24 protein, Cricetulus griseus
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Fungal Proteins
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Membrane Proteins
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Nerve Tissue Proteins
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Qa-SNARE Proteins
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R-SNARE Proteins
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SNARE Proteins
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Sec22a protein, rat
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Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
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Vesicular Transport Proteins
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Adenosine Triphosphatases
Associated data
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GENBANK/P22214
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GENBANK/U42209
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GENBANK/U91742