A Chlamydomonas homologue of the putative murine t complex distorter Tctex-2 is an outer arm dynein light chain

J Cell Biol. 1997 Jun 2;137(5):1081-90. doi: 10.1083/jcb.137.5.1081.

Abstract

Molecular analysis of a 19,000-Mr protein from the Chlamydomonas flagellum reveals that it is homologous to the t complex-encoded protein Tctex-2, which is a candidate for one of the distorter products that cause the extreme transmission ratio distortion (meiotic drive) of the murine t complex. The 19,000-Mr protein is extracted from the axoneme with 0.6 M NaCl and comigrates with the outer dynein arm in sucrose density gradients. This protein also is specifically missing in axonemes prepared from a mutant that does not assemble the outer arm. These data raise the possibility that Tctex-2 is a sperm flagellar dynein component. Combined with the recent identification of Tctex-1 (another distorter candidate) as a light chain of cytoplasmic dynein, these results lead to a biochemical model for how differential defects in spermiogenesis that result in the phenomenon of meiotic drive might be generated in wild-type vs t-bearing sperm.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Chaperonins / chemistry
  • Chaperonins / genetics
  • Chlamydomonas reinhardtii / chemistry*
  • Chlamydomonas reinhardtii / genetics
  • Cloning, Molecular
  • DNA Primers
  • DNA, Plant / analysis
  • Dyneins / chemistry
  • Dyneins / genetics*
  • Intracellular Signaling Peptides and Proteins*
  • Male
  • Mice
  • Microtubule-Associated Proteins*
  • Molecular Sequence Data
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / genetics*
  • RNA, Messenger / analysis
  • Sequence Homology, Amino Acid
  • Sperm Motility / physiology
  • Sperm Tail / chemistry
  • Ubiquitin-Protein Ligases
  • t-Complex Genome Region

Substances

  • Bacterial Proteins
  • DNA Primers
  • DNA, Plant
  • Dynlt1b protein, mouse
  • Intracellular Signaling Peptides and Proteins
  • Microtubule-Associated Proteins
  • Nuclear Proteins
  • RNA, Messenger
  • light chain protein p28, Chlamydomonas
  • PPP1R11 protein, human
  • Ubiquitin-Protein Ligases
  • Chaperonins
  • Dyneins

Associated data

  • GENBANK/U89649