Microprobe X-ray absorption spectroscopic determination of the oxidation state of intracellular chromium following exposure of V79 Chinese hamster lung cells to genotoxic chromium complexes

C T Dillon; P A Lay; M Cholewa; G J Legge; A M Bonin; T J Collins; K L Kostka; G Shea-McCarthy

doi:10.1021/tx970010m

Microprobe X-ray absorption spectroscopic determination of the oxidation state of intracellular chromium following exposure of V79 Chinese hamster lung cells to genotoxic chromium complexes

Chem Res Toxicol. 1997 May;10(5):533-5. doi: 10.1021/tx970010m.

Authors

C T Dillon¹, P A Lay, M Cholewa, G J Legge, A M Bonin, T J Collins, K L Kostka, G Shea-McCarthy

Affiliation

¹ School of Chemistry, University of Sydney, NSW, Australia.

PMID: 9168250
DOI: 10.1021/tx970010m

Abstract

The oxidation state of intracellular chromium has been determined directly in mammalian lung cells exposed to mutagenic and carcinogenic chromium compounds. Microprobe X-ray absorption spectroscopy (XAS) experiments on single V79 Chinese hamster lung cells showed that Cr(VI) and Cr(V) complexes were reduced completely (>90%) to Cr(III) within 4 h of exposure of the cells. This result provides direct evidence for the hypothesis that these genotoxic oxidants react rapidly with intracellular reductants.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Chromium / metabolism*
Chromium / toxicity
Chromium Compounds / toxicity*
Cricetinae
Cricetulus
DNA / drug effects*
Intracellular Fluid / drug effects*
Intracellular Fluid / metabolism*
Lung / cytology
Lung / drug effects*
Lung / metabolism*
Microelectrodes
Mutagenicity Tests
Oxidation-Reduction
Spectrophotometry, Atomic
X-Ray Diffraction

Substances

Chromium Compounds
Chromium
DNA