Voltage-activated sodium currents in acutely isolated mouse vestibular ganglion neurones

Neuroreport. 1997 Mar 24;8(5):1253-6. doi: 10.1097/00001756-199703240-00039.

Abstract

Voltage-activated sodium currents (INa) in vestibular ganglion neurones acutely isolated from postnatal mice were investigated using the whole-cell configuration of the patch-clamp technique. Under recording conditions designed to allow the complete isolation of INa depolarizations from a holding potential of -80 mV revealed a fast inactivating inward current which was activated around -60 mV and exhibited maximum peak current around -30 mV. This current was eliminated when the cells were perifused with a Na(+)-free solution and almost totally blocked by application of 100 nM tetrodotoxin (TTX). These properties identify this inward current as TTX-sensitive INa. The half-maximum activation potential of INa was -46 mV and its half-maximum inactivation potential was -69 mV. This is the first report of voltage-activated sodium currents in vestibular primary neurones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Electric Conductivity
  • Evoked Potentials / drug effects
  • Ganglia, Sensory / cytology
  • Ganglia, Sensory / physiology*
  • Mice
  • Neurons / physiology*
  • Patch-Clamp Techniques
  • Sodium Channels / drug effects
  • Sodium Channels / physiology*
  • Tetrodotoxin / pharmacology
  • Vestibular Nerve / cytology
  • Vestibular Nerve / physiology*

Substances

  • Sodium Channels
  • Tetrodotoxin