Identification of pore-forming subunit of P-type calcium channels: an antisense study on rat cerebellar Purkinje cells in culture

Neuropharmacology. 1997 Mar;36(3):405-9. doi: 10.1016/s0028-3908(97)00046-4.

Abstract

Treatment of cerebellar neurones in culture with an antisense oligonucleotide (ODN) against alpha1A, reduced the whole-cell P-type calcium channel current relative to mismatch ODN treated controls (p < 0.001). Therefore, AgaIVA (50 nM) reduced whole-cell calcium current in mismatch and antisense treated cells by 70 +/- 4 and 19 +/- 3%, respectively.

MeSH terms

  • Animals
  • Calcium Channels / drug effects*
  • Cells, Cultured / drug effects
  • Cerebellum / drug effects*
  • Female
  • Immunohistochemistry
  • Mice
  • Mice, Inbred BALB C
  • Oligonucleotides, Antisense / pharmacology*
  • Pregnancy
  • Purkinje Cells / drug effects*
  • Rats
  • Rats, Wistar

Substances

  • Calcium Channels
  • Oligonucleotides, Antisense