Abstract
We have cloned and sequenced a 24.5 kb region of the Bacillus subtilis 168 chromosome spanning the sspE and katA genes. The region contains a ribosomal RNA operon, rrnD, a tRNA gene set, trnD and 17 ORFs, 16 with putative ribosome-binding sites. Four of the ORFs (ORF2, ORF14, ORF16 and ORF17) match to known B. subtilis genes (sspE, thiA, senS and katA). Eight of the remaining ORF products show similarities with proteins present in the databases, including an ATP-binding transport protein, a glutamate-1-semialdehyde aminotransferase, a thiol-specific antioxidant protein, a mitomycin radical oxidase and a ferric uptake regulation protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacillus subtilis / genetics*
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Bacterial Proteins / genetics*
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Carrier Proteins / genetics
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Catalase / genetics*
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Chromosome Mapping
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Chromosomes / genetics*
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Cloning, Molecular
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Genes, Bacterial / genetics
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Intramolecular Transferases*
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Isomerases / genetics
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Molecular Sequence Data
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Open Reading Frames / genetics
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Oxidoreductases / genetics
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Peroxidases*
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Peroxiredoxins
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Proteins
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RNA, Transfer, Amino Acid-Specific / genetics
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Repressor Proteins / genetics
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Sequence Analysis, DNA
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Sigma Factor*
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Transcription Factors*
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rRNA Operon / genetics
Substances
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Bacterial Proteins
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Carrier Proteins
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Proteins
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RNA, Transfer, Amino Acid-Specific
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Repressor Proteins
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Sigma Factor
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Transcription Factors
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ferric uptake regulating proteins, bacterial
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spoIIR protein, Bacillus subtilis
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spore-specific proteins, Bacillus
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Oxidoreductases
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Peroxidases
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Peroxiredoxins
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Catalase
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KATA protein, Bacillus subtilis
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Isomerases
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Intramolecular Transferases
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glutamate-1-semialdehyde 2,1-aminomutase