Abstract
A two-hybrid screen was used to identify Saccharomyces cerevisiae genes encoding proteins that interact with MSH2. One gene was found to encode a homologue of Schizosaccharomyces pombe EXO1, a double-stranded DNA-specific 5'-3' exonuclease. S. cerevisiae EXO1 interacted with both S. cerevisiae and human MSH2 in two-hybrid and coimmunoprecipitation experiments. exo1 mutants showed a mutator phenotype, and epistasis analysis was consistent with EXO1 functioning in the MSH2-dependent mismatch repair pathway. exo1 mutations were lethal in combination with rad27 mutations, and overexpression of EXO1 suppressed both the temperature sensitive and mutator phenotypes of rad27 mutants.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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DNA Repair Enzymes
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DNA-Binding Proteins / genetics*
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Exodeoxyribonucleases / genetics*
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Exonucleases / genetics*
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Fungal Proteins*
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Genes, Fungal*
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Humans
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Molecular Sequence Data
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MutS Homolog 2 Protein
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Saccharomyces cerevisiae / genetics*
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Saccharomyces cerevisiae Proteins*
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Amino Acid
Substances
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DNA-Binding Proteins
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Fungal Proteins
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Saccharomyces cerevisiae Proteins
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Schizosaccharomyces pombe Proteins
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EXO1 protein, human
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Exodeoxyribonucleases
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Exonucleases
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exodeoxyribonuclease I
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MSH2 protein, S cerevisiae
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MutS Homolog 2 Protein
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msh2 protein, S pombe
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DNA Repair Enzymes