Expression of a kinase-defective Eph-like receptor in the normal human brain

Biochem Biophys Res Commun. 1997 Jun 27;235(3):487-92. doi: 10.1006/bbrc.1997.6812.

Abstract

We have identified a human Eph-family protein, HEP, gene located in human chromosomal region 7q33-->q35. The deduced amino acid sequence shared primary structural properties of Eph-family receptor tyrosine kinases. However, six invariant amino acids such as a lysine in the ATP-binding site and an aspartic acid in the phosphotransfer site of a conserved catalytic domain were substituted with other amino acid residues in HEP. Thus, no intrinsic tyrosine kinase activity was detectable in the catalytic domain expressed in CHO-K1 cell transfectants. Although most kinase-defective mutants of growth factor receptors have been reported as pathogenic receptors, its transcript was abundantly expressed in normal human adult tissues. A 135-kDa HEP protein was expressed in the human brain as much as in CHO-K1 cells transfected with a HEP cDNA expression vector. HEP is the first description of a kinase-defective Eph-family protein expressed abundantly in normal human tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Adult
  • Amino Acid Sequence
  • Animals
  • Aspartic Acid
  • Brain / metabolism*
  • CHO Cells
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7*
  • Conserved Sequence
  • Cricetinae
  • Drosophila Proteins*
  • Humans
  • Lysine
  • Mitogen-Activated Protein Kinase Kinases*
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Polymerase Chain Reaction
  • Protein Kinases / biosynthesis*
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism
  • Receptor Protein-Tyrosine Kinases / biosynthesis*
  • Receptor Protein-Tyrosine Kinases / chemistry
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor, EphA8
  • Receptors, Eph Family
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Reference Values
  • Transfection

Substances

  • Drosophila Proteins
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • Aspartic Acid
  • Adenosine Triphosphate
  • Protein Kinases
  • EPHB6 protein, human
  • Protein-Tyrosine Kinases
  • Receptor Protein-Tyrosine Kinases
  • Receptor, EphA8
  • Receptors, Eph Family
  • Hep protein, Drosophila
  • Mitogen-Activated Protein Kinase Kinases
  • Lysine

Associated data

  • GENBANK/D83492
  • GENBANK/U07695
  • GENBANK/X03663