Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa

W H Haas; K Schilke; J Brand; B Amthor; K Weyer; P B Fourie; G Bretzel; V Sticht-Groh; H J Bremer

doi:10.1128/AAC.41.7.1601

Molecular analysis of katG gene mutations in strains of Mycobacterium tuberculosis complex from Africa

Antimicrob Agents Chemother. 1997 Jul;41(7):1601-3. doi: 10.1128/AAC.41.7.1601.

Authors

W H Haas¹, K Schilke, J Brand, B Amthor, K Weyer, P B Fourie, G Bretzel, V Sticht-Groh, H J Bremer

Affiliation

¹ Department of General Pediatrics, Children's Hospital, Heidelberg University, Germany. [email protected]

Abstract

A sample of 124 isoniazid (INH)-resistant and 88 susceptible strains of Mycobacterium tuberculosis complex from south, central, and west Africa was analyzed by direct sequence analysis and PCR-restriction fragment length polymorphism analysis of their catalase-peroxidase (katG) genes. Point mutations at codon 315 were found in the genomes of 64% of INH-resistant strains, but no complete deletions were identified. Mutations at codon 463 were independent of INH resistance and were linked to the geographic origins of the strains.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Africa
Catalase / genetics*
Genes, Bacterial*
Molecular Sequence Data
Mycobacterium tuberculosis / genetics*
Peroxidase / genetics*
Point Mutation*

Substances

Catalase
Peroxidase

Associated data

GENBANK/X68081