Ultrastructure of rat Purkinje neurons after chronic ethanol consumption and prolonged abstinence

Anat Histol Embryol. 1997 Jun;26(2):93-8. doi: 10.1111/j.1439-0264.1997.tb00106.x.

Abstract

In the present study of nutrition control of Wistar white rats, the ultrastructure of cerebellar Purkinje cells was studied after chronic ethanol exposure and a subsequent period of prolonged abstinence: a qualitative investigation of the perikarya of Purkinje cells was performed in age-matched controls (group A) and rats alcohol-fed for 5 months and withdrawn from this diet for 3 months (group B). The results showed massive accumulation of small dense bodies as well as obvious deposition of lipofuscin in the Purkinje cells of group B. Furthermore, ring-shaped Golgi apparatus units, lamellar bodies and degenerative foci dispersed throughout the cytoplasm of the alcohol-treated animals referred to degeneration processes and neuronal cell death, the morphological characteristics and the aetiology of which are discussed.

MeSH terms

  • Alcohol Drinking / physiopathology*
  • Animals
  • Cytoplasm / ultrastructure
  • Dose-Response Relationship, Drug
  • Ethanol / administration & dosage
  • Ethanol / pharmacology*
  • Golgi Apparatus / ultrastructure
  • Lipofuscin / analysis
  • Male
  • Microscopy, Electron / methods
  • Microscopy, Electron / veterinary
  • Nerve Degeneration / physiology
  • Neurons / chemistry
  • Neurons / drug effects
  • Neurons / ultrastructure*
  • Organelles / ultrastructure
  • Purkinje Cells / chemistry
  • Purkinje Cells / drug effects
  • Purkinje Cells / ultrastructure*
  • Rats
  • Rats, Wistar

Substances

  • Lipofuscin
  • Ethanol