Arteriolar dilation produced by venule endothelium-derived nitric oxide

Microcirculation. 1997 Jun;4(2):303-10. doi: 10.3109/10739689709146793.

Abstract

Objective: We conducted bioassay experiments to determine whether nitric oxide produced by endothelial cells (endothelial-derived nitric oxide, or EDNO) within large venules could act to dilate arterioles.

Methods: In these experiments parallel segments of first-order arterioles and venules were isolated from skeletal muscle and were cannulated in series with a glass connecting tube (length: 300-500 microns). Arterioles were mechanically denuded of endothelium by a delicate yet abrasive rubbing technique. Venular endothelium remained intact. Endothelial denudation of arterioles was confirmed by the absence of dilation during exposure to acetylcholine (10(-6) mol/L). The cannulated vessels were pressurized to 30 cm H2O and the arterioles pre-constricted by approximately 50% with norepinephrine (10(-10) mol/L).

Results: Topical applications of acetylcholine (10(-6) mol/L) or bradykinin (10(-9) mol/L) during luminal perfusion from venule to arteriole produced significant arteriolar dilation. In contrast, a slight arteriolar constriction was observed when the direction of flow was reversed (i.e., arteriole to venule) in the presence of either acetylcholine (10(-6) mol/L) or bradykinin (10(-9) mol/L). Inhibition of venular EDNO with NG-monomethyl-L-arginine (L-NMMA; 10(-5) mol/L; 1 hour) completely abolished the arteriolar dilation observed in response to acetylcholine or bradykinin during venule to arteriole perfusion.

Conclusions: These results demonstrate that venular-derived EDNO can relax arteriolar vascular smooth muscle.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine / metabolism
  • Adenosine / pharmacology
  • Animals
  • Arterioles / drug effects*
  • Arterioles / physiology*
  • Endothelium, Vascular / physiology*
  • Male
  • Microcirculation / drug effects
  • Microcirculation / physiology
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / physiology
  • Nitric Oxide / metabolism
  • Nitric Oxide / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Regional Blood Flow / drug effects
  • Vasodilation* / drug effects
  • Venules / physiology*

Substances

  • Nitric Oxide
  • Adenosine