Facilitated oxygen transport with modified and encapsulated hemoglobins across non-flowing solution membrane

Artif Cells Blood Substit Immobil Biotechnol. 1997 Jul;25(4):335-46. doi: 10.3109/10731199709118924.

Abstract

The oxygen-transporting capability of modified and encapsulated hemoglobins and red cells is discussed from a physico-chemical standpoint in order to design oxygen-delivering fluids. The oxygen diffusion coefficient toward oxygen-deficient sites was estimated by measuring the oxygen flux across thin solution membranes of hemoglobin, polymerized hemoglobin, liposome-encapsulated hemoglobin, and red cells. Oxygen flux was enhanced several times over that of nitrogen for the hemoglobin and red cell solution with ca [Hb] = 10 and 15 g/dl, respectively. The enhancement in the oxygen diffusion is ascribed to the facilitated transport of oxygen via the hemoglobins. This was in contrast to the simple and physical oxygen-diffusivity in response to its concentration gradient, in the absence of hemoglobins. The flux of the oxygen transport was in the order of hemoglobin > red cells > polymerized hemoglobin > encapsulated hemoglobin, which was ascribed to the facilitated transport efficiencies of oxygen with hemoglobins in a non-flowing or stationary solution.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Blood Substitutes / metabolism*
  • Cattle
  • Erythrocyte Membrane / metabolism
  • Hemoglobins / metabolism*
  • Humans
  • Liposomes / metabolism
  • Membranes, Artificial*
  • Models, Biological
  • Oxygen / blood*
  • Solutions

Substances

  • Blood Substitutes
  • Hemoglobins
  • Liposomes
  • Membranes, Artificial
  • Solutions
  • Oxygen