Abstract
2'-Phospho-cyclic ADP-ribose (P-cADPR) is a newly identified Ca2+-mobilizing agent derived from NADP that stimulates intracellular Ca2+ release by a mechanism distinct from inositol 1, 4, 5-trisphosphate. In this report, we show that P-cADPR is an endogenous metabolite in bovine tissues with basal levels ranging from 17.6 to 89.5 fmol/mg protein. The natural occurrence of this Ca2+-mobilizing nucleotide provides a potential link between NADP(H) metabolism and regulation of Ca2+ homeostasis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Diphosphate Ribose / analogs & derivatives*
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Adenosine Diphosphate Ribose / analysis
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Animals
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Brain Chemistry
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Calcium / metabolism*
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Cattle
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Cyclic ADP-Ribose* / analogs & derivatives*
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Kidney / chemistry
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Liver / chemistry
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Lung / chemistry
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Myocardium / chemistry
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NADP / metabolism*
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Organ Specificity
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Radioimmunoassay
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Reference Values
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Spleen / chemistry
Substances
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cyclic ADP-ribose 2'-phosphate
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Cyclic ADP-Ribose
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Adenosine Diphosphate Ribose
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NADP
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Calcium