Abstract
The CCAAT box is an important element in eukaryotic promoters and NF-Y (CBF) is a conserved heterotrimeric protein binding to it. Two subunits, NF-YB and NF-YC, contain a histone-like motif. We cloned the complete cDNA coding for the human NF-YC gene. The ORF codes for a 335 aa protein that shows virtual identity to the rat sequence, confirming the stunning invariance of NF-Y genes across species. We expressed and purified the yeast homology domain of NF-YC in bacteria and performed EMSA together with the corresponding conserved domains of NF-YA and NF-YB, obtaining a CCAAT-binding mini-NF-Y. We evaluated the expression of NF-YC and found that mRNA levels are similar in different human tissues except in testis.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Binding Sites
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Biological Evolution
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CCAAT-Enhancer-Binding Proteins
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Cloning, Molecular
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Conserved Sequence
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DNA, Complementary / genetics
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism
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Escherichia coli / genetics
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Humans
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Molecular Sequence Data
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Peptide Fragments / genetics
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Peptide Fragments / isolation & purification
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Peptide Fragments / metabolism
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Protein Binding
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Protein Conformation
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RNA, Messenger / genetics
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Sequence Analysis, DNA
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Species Specificity
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Tissue Distribution
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Peptide Fragments
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RNA, Messenger
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Recombinant Proteins