Abstract
Sequence analysis is a powerful tool to obtain structural and functional information about genes and their products. Here we show that TSG101, a gene subjected to somatic mutations in breast cancer, contains an amino terminal domain that is a homologue of ubiquitin conjugating enzymes (UBCs) and not, as previously proposed, DNA-binding domains. As the UBC active site residue is replaced in the TSG101 sequence in a similar manner to several other members of the UBC family, we propose a role for TSG101 in regulating the ubiquitination of short-lived gene products.
MeSH terms
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Amino Acid Sequence
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Animals
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Breast Neoplasms / enzymology
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Breast Neoplasms / genetics*
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Chickens
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / physiology*
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Endosomal Sorting Complexes Required for Transport
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Female
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Humans
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Ligases / chemistry
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Ligases / genetics
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Mice
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Molecular Sequence Data
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Saccharomyces cerevisiae
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Sequence Homology, Amino Acid
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / physiology*
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Ubiquitin-Conjugating Enzymes
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Ubiquitins / metabolism*
Substances
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DNA-Binding Proteins
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Endosomal Sorting Complexes Required for Transport
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Transcription Factors
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Tsg101 protein
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Ubiquitins
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Ubiquitin-Conjugating Enzymes
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Ligases
Associated data
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GENBANK/AA199318
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GENBANK/L77699
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GENBANK/U13644
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GENBANK/U39360
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GENBANK/U39361
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GENBANK/U39674
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GENBANK/U62136
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GENBANK/U82130
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GENBANK/U97571
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GENBANK/X71978
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GENBANK/Z86109
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SWISSPROT/P06104
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SWISSPROT/P14682
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SWISSPROT/P15731
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SWISSPROT/P15732
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SWISSPROT/P21734
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SWISSPROT/P25604
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SWISSPROT/P28263
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SWISSPROT/P29340
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SWISSPROT/P33296
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SWISSPROT/P50623
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SWISSPROT/P52490
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SWISSPROT/P52491
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SWISSPROT/P52492
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SWISSPROT/P53152
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SWISSPROT/Q02159