Abstract
We found that CPY production in Saccharomyces cerevisiae KS58-2D/pCY303 was increased by the addition of thiamine into the medium, while the addition of thiamine had no effect on cell growth. It became clear that the positive effect of thiamine was due to transcriptional increase, because the levels of CPYmRNA were increased according to the amount of thiamine added. Furthermore, it was suggested that thiamine generally increases the expression of yeast genes, since the expression of the luciferase gene that was artificially constructed was also increased to some extent by thiamine in S. cerevisiae.
MeSH terms
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Actins / drug effects
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Actins / genetics
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Actins / metabolism
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Arabidopsis Proteins*
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Carboxypeptidases / drug effects
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Carboxypeptidases / genetics*
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Carboxypeptidases / metabolism
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Cathepsin A
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Extracellular Matrix Proteins / drug effects
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Extracellular Matrix Proteins / genetics
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Extracellular Matrix Proteins / metabolism
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Gene Expression Regulation, Fungal / drug effects*
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Luciferases / drug effects
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Luciferases / genetics
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Luciferases / metabolism
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RNA, Messenger / biosynthesis
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RNA, Messenger / drug effects
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / genetics*
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Thiamine / pharmacology*
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Transcription, Genetic
Substances
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AAC1 protein, Arabidopsis
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Actins
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Arabidopsis Proteins
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Extracellular Matrix Proteins
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RNA, Messenger
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Luciferases
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Carboxypeptidases
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Cathepsin A
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Thiamine