How to reconstruct the damaged articular cartilage has been one of the main subjects in treatment of articular disease for a long time. In this experimental study, fullthickness cartilage defects of 4 mm x 5 mm in size were created in rabbit's TMJ condyle cartiage on the left sides. The autogenous free periosteum were transplanted to the defects by using biological gelatin. Postoperatively, the animals were killed at intervals of 1, 2, 4, 8, 12 and 16 weeks. The regenerative cartilage in the defects were examined by gross appearance and histology. The result showed that mesenchymal cells in the cambium layer of periostum can transform into chondroblasts under the mechanical stress.