A transgenic mouse strain expressing four drug-selectable marker genes

Nucleic Acids Res. 1997 Sep 15;25(18):3745-6. doi: 10.1093/nar/25.18.3745.

Abstract

Murine embryonic stem (ES) cells are commonly cultured on feeder layers of primary murine embryonic fibroblasts (MEFs). Because gene targeting experiments often involve sequential selection for multiple-drug resistance in single ES cell lines, we have developed a new mouse strain which represents an economical donor for the production of multiple-drug resistant MEFs. MEFs prepared from the DR-4 mouse strain displayed resistance to concentrations of the drugs G418, 6-thioguanine, puromycin and hygromycin well above those used normally for the selection of drug-resistant ES cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Biomarkers
  • Drug Resistance, Multiple / genetics*
  • Fibroblasts
  • Mice
  • Mice, Transgenic*

Substances

  • Biomarkers