Abstract
Ultraviolet (UV) A irradiation of human dermal fibroblasts elicits an increase in specific mRNA amounts and bioactivities of the cytokines IL-1alpha, IL-1beta, and IL-6. These effects are enhanced in deuterium oxide-based medium and are diminished in the presence of non-toxic concentrations of sodium azide. Furthermore, generating singlet oxygen outside the cells by irradiation of rose bengal-coated resin particles with visible light (lambda > 450 nm) results in the induction of interstitial collagenase, IL-1 and IL-6, similar to the response observed with UVA irradiation. These observations suggest that singlet oxygen is an early intermediate in the signaling pathway of IL-1 and IL-6 mediating UVA induction of interstitial collagenase (E.C. 3.4.24.7). Furthermore, singlet oxygen appears to initiate this complex UV response at the cell membrane.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Azides / pharmacology
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Cells, Cultured
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Collagenases / genetics*
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Fibroblasts / physiology*
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Gene Expression Regulation, Enzymologic / drug effects
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Gene Expression Regulation, Enzymologic / physiology*
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Gene Expression Regulation, Enzymologic / radiation effects
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Humans
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Interleukin-1 / genetics
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Interleukin-1 / metabolism
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Interleukin-6 / genetics
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Interleukin-6 / metabolism
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Interleukins / genetics*
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Interleukins / metabolism
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Matrix Metalloproteinase 1
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Naphthols / pharmacology
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Oxygen / physiology*
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RNA, Messenger / analysis
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Rose Bengal / pharmacology
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Signal Transduction / physiology
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Singlet Oxygen
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Skin / cytology
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Sodium Azide
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Ultraviolet Rays
Substances
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Azides
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Interleukin-1
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Interleukin-6
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Interleukins
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Naphthols
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RNA, Messenger
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Singlet Oxygen
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Rose Bengal
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Sodium Azide
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3,3'-(1,4-naphthylidene)diproprionate
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Collagenases
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Matrix Metalloproteinase 1
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Oxygen